Royer Group: Organismal Architecture

How do organisms develop from a single cell into a fully functional body, with billions of self-organizing cells that form tissues and have different functions?

Our Research

We are a pluridisciplinary team of computer scientists, optical engineers, and biologists.

Our goal is to build a time-resolved, multidimensional atlas of vertebrate development using zebrafish as a model organism.

To attain this goal, we design, build, and implement novel state-of-the-art light-sheet microscopes, and employ and also design deep learning–based image processing and analysis algorithms.

Light-sheet microscope used by the Royer Group for zebrafish imaging

Bioimage Informatics

Deep learning-based image restoration, 5D bioimage visualization, GPU-accelerated image processing

Custom light-sheet microscopy instrument built by the Royer Group

Advanced Microscopy

Light-sheet microscopy, single objective microscopy, adaptive imaging, smart microscopy

Zebrafish embryo imaged for developmental biology research

Developmental Biology

Cellular atlas of vertebrate development, Zebrafish development

Selected Papers and Projects

Zebrahub

Zebrahub thumbnail showing a multidimensional zebrafish development atlas

bioRxiv, 2024
Kim et al.

Cell, 2024
Lange et al.

Adaptive Imaging

Adaptive imaging technique adjusting microscope focus in real time

Image Restoration

Deep learning-based image restoration applied to microscopy data

Nature Methods, 2018
Weigert et al.

Nature Methods, 2019
Beltangady and Royer

PMLR, 2019
Batson and Royer

Light-Sheet Microscopy

Light-sheet microscopy setup capturing a developing zebrafish embryo

Bioimage Visualization

5D bioimage visualization interface displaying volumetric cell data

bioRxiv, 2024
Huijben et al.

Nature Methods, 2015
Royer et al.

Image Processing & Analysis

GPU-accelerated image processing pipeline for large bioimaging datasets

bioRxiv, 2024
Bragantini et al.

Nature Methods, 2022
Kobayashi et al.

Nature Methods, 2019
Haase et al.

Open-Source Hardware

Open-source microscopy hardware component designed by the Royer Group

eLife, 2021
Lange et al.

napari

Napari open-source viewer displaying multidimensional bioimage data

Created by Loïc Royer, Juan Nuñez-Iglesias of Monash University, and Nick Sofroniew of the Chan Zuckerberg Initiative, napari is an open-source collaboration involving Biohub, the CZI Science Technology team, and many other scientists and computational biologists around the world.

Lab Gallery